Part:BBa_I714891:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I714891

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  measured by fluorescence microscope

http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai team conducted split EGFP assay which could readily help visualize protein interaction. [BBa_K771113] [BBA_K771114]

[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] provided specific characterization for this part by using eGFP for experimental characterization of lentiviral transfer plasmid transfection efficacy and apoptotic effect on colorectal cancer cell line RKO.

edit by sysu-medicine

We validated this part by insert it and CMV promoter together into the vertor. The map of the plasmid is as followed(Fig.1). Then we transfected the plasmid to 293T cells, the expression of eGFP protein was observed by fluorescence microscope(Fig.2) and quantified by Image J(Fig.3). You can also see the characterization of this part in our composite part BBa_K2942705, BBa_K2942706, BBa_K2942707, BBa_K2942708.

Fig.1 The plasmid we used for transfection.

Fig.2 Transfection cells observed under a fluorescence microscope.

Fig.3 Fluorescent quantitation by Image J(P<0.001).

UNIQ408100efc27cad7d-partinfo-00000001-QINU

BBa_I714891 Tsinghua-D

This part works well for providing eGFP in high expression. We improve this part by adding a sequence of RNAT in from the eGFP gene. Thus, the expression of the reporter gene can be regulated by sensing the temperature.

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UNIQ408100efc27cad7d-partinfo-00000003-QINU